G- & C-bands protocol

1 Stain mitotic cells 5 minutes in 4% Gurr Giemsa in Gurr buffer (for bone marrow).

• 2 ml Karyomax Giemsa (Gurr's Improved R66 solution #10092-013)
• 48ml Gurr buffer pH 6.8 (buffer tabs #10582-013)
• Rinse under cool running tap water followed by several rinses of distilled water.

2 Stain meiotic cells 5 minutes as above except when doing testis preparations use Fisher stain.

• 2ml Fisher Giemsa (#SG28-475)
• 48ml PO4 buffer pH 7.0 (#SB108-20) or Gurr buffer, pH 6.8(# 10582-013)
• Rinse under cool running tap water followed by distilled water.

Quick G-banding

1. Place slides in 60-65°C incubator overnight.
2. Stain in 2.2% Giemsa with 4 qtts 0.0125 trypsin (1mlGiemsa / 45ml Gurr buffer)
3. Leave 4-6 mins

Quick staining

1. 1ml Gurr Geimsa
2. 45ml Gurr buffer
3. Leave 4-5 mins